Rapid detection of listeria monocytogenes in food with multiplex - nested pcr 檢測食品中單增李斯特菌研究
Study of the nested pcr detection salmonella typhi in the blood specimens 巢式聚合酶鏈反應(yīng)快速檢測血液標(biāo)本中
Definition of nested pcr 巢式pcr的定義
By using the method of genome walking , two fragments were cloned from genome walker library after the semi - nested pcr . the result of sequencing the bigger fragment showed that this 706bp sequence contained the atg start codon 通過半巢式的基因步移法,經(jīng)二輪pcr后,從genomewalker文庫分離到2個片段,較大的片段測序表明該706bp序列含有編碼ast的起始密碼子atg ,其上游序列為啟動子序列。
nested pcr meaning:[Medicine] In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The ess...